Informatics Publishing Limited

Krushna H. Pande ¹, Pramod S. Salve ¹, Raghvendra K. Rai ¹, Nikhil R. Bali ¹, Harshad S. Wankhede ²

Affiliations
1 University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440033, (M.S), IN
2 University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440033, (M.S)

Abstract

Diabetes mellitus, a metabolic disorder, is becoming the third "killer" of the health of mankind along with cancer, cardiovascular and cerebrovascular diseases because of its high prevalence, morbidity and mortality. Medicinal plants play an important role in the treatment of diabetes mellitus, particularly in the developing countries due to their low cost. Aegle marmelos popularly known as bael, golden apple and Withania coagulans known as Indian cheese maker, panir dodi, panir ka ful, are commonly used as a food and in the Indian traditional medicinal system for the management various diseases particularly type 2 diabetic mellitus. The present investigation was conducted to evaluate the potential synergistic anti-diabetic activity of combined extract of Aegle marmelos and Withania coagulans. Fresh leaves of Aegle marmelos and dried fruits of Withania coagulans were used in the study. Maceration with timely stirring process and de-fatting method were employed for the preparation of aqueous extracts. Alloxan-induced diabetic animal model was used for evaluation of pharmacological activity. Acute antidiabetic study (24 hours) in Alloxan-induced diabetic mice treated with standard drug glibenclamide, 31.90% maximum fall in fasting blood glucose (FBG) level was observed while combined extract (AM+WC3) shown 23.35% maximum fall in FBG. Long term sub-chronic anti-diabetic evaluation (21 Days) demonstrate the impact of the most effective combination extract (AM+WC2) which lowered 50.29% FBG on 21^th day while glibenclamide exerted only 39.11% maximum fall in FBG. Results from acute and sub-chronic antidiabetic studies clearly shows the reliability of this novel synergistic combination approach of these traditional herbs extracts for efficient, long term, static and therapeutically safe management of prevailing diabetic condition.

Keywords

Diabetes Mellitus, Aegle marmelos, Withania coagulans , Alloxan-Induced Diabetic Animal Model, Glibenclamide.

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Pramod S. Salve ¹, Nikhil Bali ¹, Navleen Saini ¹

Affiliations
1 University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440033, (M.S), IN

Abstract

Coronary artery diseases (CAD) represent a condition in which the blood supply to the heart muscle is partially or completely blocked. In atherosclerosis, the hardening of arteries occurs with decreasing elasticity of the arteries. For management of disease, cholesterol lowering agents like Pravastatin and anti-platelet agent like aspirin are commonly used. Various studies have shown that these agents when given in combination results in synergistic pharmacological activity, however they are not chemically compatible. In present work, formulation approaches utilizes principles of pelletization and coating technologies to form a stable drug delivery system. The enteric coating of the core aspirin pellets was done using Eudragit L 100 (6%) with polyethylene glycol 6000 (0.6%) till 2.5, 5 and 7.5 % weight gain was obtained. A barrier coating on enteric coated pellets was applied using hydroxyl propyl methyl cellulose (HPMC) 15 cps solution. For the layering of Pravastatin over the barrier coated pellets, Pravastatin was dispersed in 2.5% HPMC solution. The coating solution was sprayed over the pellets till a dose strength equivalent to 10 mg of Pravastatin per 250 mg of pellets was obtained. The optimized pellets were subjected to evaluation of drug content, bulk density, crushing strength, friability, size distribution, packing ability and in-vitro drug release, stability studies and in-vivo performance using trition X 100 induce hyperlipidaemia rat model. The developed formulation showed an effective cholesterol lowering effect as compared to standard marked formulation of Pravastatin. It indicates the development of stable combination formulation in which aspirin forms the enteric coated sustained release core and that of Pravastatin fast releasing outer layers of the pellets.

Keywords

Atherosclerosis, Pravastatin, Aspirin, Pelletization, Trition X 100 Induce Hyperlipidaemia Rat Model.

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